Fusidic acid resistance and fitness in S. aureus
نویسندگان
چکیده
Background: Fitness loss and compensation in fusidic acid resistant EF-G mutants of Staphylococcus aureus. Results: Slower translocation and ribosomerecycling due to restricted conformational change, plus increased tRNA drop-off cause fitness loss in F88L; these are recovered in F88L+M16I leading to fitness compensation. Conclusion: Conformational dynamics of EF-G is crucial for its function. Significance: Provides insight into how antibiotic resistant mutations affect in vivo fitness.
منابع مشابه
Compensatory adaptation to the loss of biological fitness associated with acquisition of fusidic acid resistance in Staphylococcus aureus.
Recent studies have shown that individual amino acid exchanges within elongation factor G (EF-G) cause fusidic acid resistance in Staphylococcus aureus. The data from the present study illustrate that the fusidic acid resistance-mediating amino acid substitutions P406L and H457Y are associated with a marked impairment of the biological fitness of S. aureus. In particular, strains producing EF-G...
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